virus strains encephalomyocarditis virus emcv atcc  (ATCC)

Journal: EMBO Reports

Article Title: Cholesterol restriction primes antiviral innate immunity via SREBP1-driven noncanonical type I IFNs

doi: 10.1038/s44319-024-00346-9

Figure Lengend Snippet: Reagents and tools table

Article Snippet: EMCV , ATCC , VR-1762 strain.

Techniques: Recombinant, Sequencing, CRISPR, Knock-Out, Cloning, Plasmid Preparation, Membrane, Protease Inhibitor, Software, Virus, Reporter Assay, Chromatin Immunoprecipitation, Magnetic Beads, Enzyme-linked Immunosorbent Assay

Journal: Journal of Virology

Article Title: A rapid and versatile reverse genetics approach for generating recombinant positive-strand RNA viruses that use IRES-mediated translation

doi: 10.1128/jvi.01638-23

Figure Lengend Snippet: Generation of the recombinant EMCV (class II IRES). (A) A schematic representation of the EMCV genome and the gene fragments prepared. A total of six fragments were amplified and then assembled with the new UTR linker fragment by CPER. Gel electrophoresis analysis was conducted to confirm the size of the six PCR amplicons (left panel). PCR assembly was used to connect neighboring fragments and the size of joined fragments was assessed by gel electrophoresis (right panel). F: fragment; M: 1 kb DNA ladder. (B) Huh7 cells were inoculated with 100 µL of culture supernatants collected from CPER-transfected cells. At 8 hpi, the cells were subjected to immunofluorescent staining of double-stranded RNA (dsRNA; green). Nuclei were counterstained with DAPI (blue). Scale bars, 25 µm. (C) Huh7 cells were inoculated with recombinant EMCV at an MOI of 0.01. The virus titers in supernatants and intracellular viral RNA were determined at the indicated timepoints by plaque-forming assay and qRT-PCR, respectively. (D) Both linkers were individually subjected to CPER assembly to evaluate the efficiency of recovery of recombinant EMCV as measured by a plaque-forming assay (bar graph). Asterisks indicate significant differences (*P < 0.05) versus the results of the CMV promoter. ND: not detected. Assays were performed independently in triplicate (C, D).

Article Snippet: The cDNA EMCV VR-129B strain (GenBank accession number: KM269482 ) was synthesized by Thermo Fisher Scientific.

Techniques: Recombinant, Amplification, Nucleic Acid Electrophoresis, Transfection, Staining, Virus, Quantitative RT-PCR

Journal: Cell Reports

Article Title: The HIF transcription network exerts innate antiviral activity in neurons and limits brain inflammation

doi: 10.1016/j.celrep.2024.113792

Figure Lengend Snippet: HIF activation exerts broad antiviral activity in neuron-like cells (A and B) Virus titer of vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV) in culture supernatants from SH-Sy5y cells treated with ML228 (0.5 μM) and infected for 19 h with EMCV (MOI 0.1) or VSV (MOI 0.001). n = 3 biological replicates. (C) SARS-CoV-2 N2 RNA levels evaluated by RT-qPCR in total RNA from SH-Sy5y cells treated with ML228 (0.5 μM) and infected for 48 h with SARS-CoV-2 (MOI 1). Data were normalized against 18S rRNA. n = 5 biological replicates. (D) Influenza A virus M2 RNA levels evaluated by RT-qPCR in total RNA from SH-Sy5y cells treated with ML228 (0.5 μM) and infected for 24 h with influenza A virus (MOI 0.1). Data were normalized against 18S rRNA. n = 5 biological replicates. (E) Measles virus titer in culture supernatants from SH-Sy5y cells treated with ML228 (0.5 μM) and infected for 36 h with Measles virus (MOI 0.1). n = 6 biological replicates. Data are representative of at least three independent experiments. The data are shown as means ± SD. Individual values are represented by dots. For (A), Mann-Whitney was applied to identify statistical significance. For (B)–(E), the statistical analyses were performed by Student’s t test, two-tailed, parametric distribution to identify statistical significance. ns, not significant. Illustrations of viruses were generated with BioRender.

Article Snippet: EMCV (EMC) strain , ATCC , #VR-129B.

Techniques: Activation Assay, Activity Assay, Virus, Infection, Quantitative RT-PCR, MANN-WHITNEY, Two Tailed Test, Generated

Journal: Cell Reports

Article Title: The HIF transcription network exerts innate antiviral activity in neurons and limits brain inflammation

doi: 10.1016/j.celrep.2024.113792

Figure Lengend Snippet:

Article Snippet: EMCV (EMC) strain , ATCC , #VR-129B.

Techniques: Virus, Variant Assay, Recombinant, Isolation, Extraction, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Sample Prep, Expressing, Software, Imaging, Modification, Knock-Out